Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Targeting PEG10 as a novel therapeutic approach to overcome CDK4/6 inhibitor resistance in breast cancer

doi: 10.1186/s13046-023-02903-x

Figure Lengend Snippet: Ectopic overexpression of PEG10 augments EMT and leads to palbociclib resistance. A Immunoblot demonstrates ectopic overexpression of different PEG10 protein isoforms in MCF7 and T47D cells. Ectopic overexpression of PEG10 elevated the ZEB1 and suppressed the E-cadherin expression. B , C Representative images from a migration and invasion assay after ectopic overexpression of different PEG10 protein isoforms in MCF7 and T47D cells. The area of migratory and invading cells from three different non-overlapping 100 × microscopic fields is expressed as mean ± SD in the right panel. Independent sample t-test: * p < 0.05, ** p < 0.01, *** p < 0.001. D - I Cell viability (MTT) assay of MCF7 and T47D cells before and after ectopic overexpression of PEG10-RF1 and subsequent treatment with the indicated dose of palbociclib, abemaciclib, and ribociclib for 72 h. Three independently repeated experiments were performed with similar results. Independent sample t-test: * p < 0.05, ** p < 0.01, *** p < 0.001, Abbreviation: ns, not significant. J , K Cell cycle distribution of MCF7 and T47D cell line before and after the ectopic overexpression of different PEG10 protein isoforms and subsequent treatment with the IC 50 concentration of palbociclib for 48 h. Three independently repeated experiments were performed with similar results. Independent sample t-test: ** p < 0.01, Abbreviation: ns, not significant

Article Snippet: Palbociclib (cat# CS-0019), abemaciclib (cat# CS-1230) and ribociclib (cat# CS-1750) were purchased from ChemScene (Monmouth Junction, NJ, USA).

Techniques: Over Expression, Western Blot, Expressing, Migration, Invasion Assay, MTT Assay, Concentration Assay

Journal: bioRxiv

Article Title: Brain map of aging-induced alterations in membrane ganglioside pattern

doi: 10.1101/2023.08.30.555520

Figure Lengend Snippet: Effect of aging on the rat brain GSs levels in individual rat brain structures. a–i Changes in GSs levels ( a GM1, b GD1a, c GT1b, d GD3, e GD1b, f GQ1b, g GD2, h GM3, i GM2); ordered by abundance) expressed as µg of GSs per mg of brain tissue DW. The levels were determined in 1 month and 11 months aged groups in the neocortex (NEO), allocortex (ALLO), cerebellum (CB), olfactory bulbs (OB), medial basal forebrain (MF), hypothalamus (HYPO), brainstem (BS), cingulate cortex (CING), striatum (STR), ventral (VHIP) and dorsal hippocampus (DHIP). Data were analyzed using an adjusted F test with Kenward-Roger type adjustment of denominator degrees of freedom. n = 13 (NEO 12) for the group of 1-month-old rats and n = 13 (DHIP 12) for 11 months old rats. Statistical significance is indicated with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. The boxes span from the 25th to the 75th percentiles, the lines in the middle denote the median values, and the whiskers extend from the smallest values and up to the largest values (dots show all data points).

Article Snippet: GM1 standard (cat. #345724) was from Calbiochem (USA), GM2 (cat. #1502), and GD2 (cat. #1527) from Matreya (Pleasant Gap, PA).

Techniques:

Journal: bioRxiv

Article Title: Brain map of aging-induced alterations in membrane ganglioside pattern

doi: 10.1101/2023.08.30.555520

Figure Lengend Snippet: Bar chart depicting the a-series to b-series GSs in the brain regions. The a-series GS included GM1, GM2, GD1a, and GM3, whereas the b-series included GD1b, GT1b, GQ1b, GD2, and GD3. Statistical significance is indicated with *p ≤ 0.05. The regions with the starkest changes during neurodevelopment were the hippocampus, hypothalamus, medial forebrain, and brainstem.

Article Snippet: GM1 standard (cat. #345724) was from Calbiochem (USA), GM2 (cat. #1502), and GD2 (cat. #1527) from Matreya (Pleasant Gap, PA).

Techniques: